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A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method.
Favero, Vivian; Veríssimo, Carolina De Marco; Piovesan, Angela R; Morassutti, Alessandra L; Souto, André A; Bittencourt, Hélio R; Pascoal, Vanessa F; Lindholz, Catieli G; Jones, Malcolm K; Souza, Renata P; Rigo, Francine De Vargas; Carlini, Célia R; Graeff-Teixeira, Carlos.
Afiliação
  • Favero V; Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  • Veríssimo CM; Neurotoxins Laboratory (LaNeuroTox), School of Medicine, Brain Institute and School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  • Piovesan AR; Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  • Morassutti AL; Centre for One Health and Ryan Institute, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
  • Souto AA; Neurotoxins Laboratory (LaNeuroTox), School of Medicine, Brain Institute and School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  • Bittencourt HR; Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Pascoal VF; Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  • Lindholz CG; Polytechnic School, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  • Jones MK; Polytechnic School, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  • Souza RP; Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  • Rigo FV; Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
  • Carlini CR; School of Veterinary Science, The University of Queensland, Brisbane, Qld, Australia, 4072.
  • Graeff-Teixeira C; Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
PLoS Negl Trop Dis ; 14(7): e0008500, 2020 07.
Article em En | MEDLINE | ID: mdl-32730339
ABSTRACT
Schistosomiasis remains a serious public health problem in tropical regions, affecting more than 250 million people. Sensitive diagnostic methods represent key tools for disease elimination, in particular in areas with low endemicity. Advances in the use of luminol-based chemiluminescent techniques have enabled greater sensitivity and speed in obtaining results in different diagnostic settings. In this study, we developed a luminol-H2O2 chemiluminescence (CL) method to detect Schistosoma mansoni eggs in human fecal sediments processed by the Helmintex (HTX) method. After S. mansoni eggs were incubated with a solution of luminol-H2O2 the light emission was detected and measured by spectrophotometry at 431 nm for 5 min, using detection and counts of eggs by bright field optical microscopy as a reference. CL intensity was found to correlate with different sources and numbers of eggs. Furthermore, our results showed that the CL method can distinguish positive from negative samples with 100% sensitivity and 71% specificity. To our knowledge, this is the first study to report the use of CL for the diagnosis of helminths from fecal samples. The combination of the HTX method with CL represents an important advance in providing a reference method with the highest standards of sensitivity.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Óvulo / Schistosoma mansoni / Esquistossomose mansoni / Fezes / Peróxido de Hidrogênio / Luminol Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Óvulo / Schistosoma mansoni / Esquistossomose mansoni / Fezes / Peróxido de Hidrogênio / Luminol Idioma: En Ano de publicação: 2020 Tipo de documento: Article