Sequential modifications of glycans by linkage-specific alkylamidation of sialic acids and permethylation.
Anal Biochem
; 606: 113861, 2020 10 01.
Article
em En
| MEDLINE
| ID: mdl-32739348
ABSTRACT
Permethylation is useful for glycosidic linkage analysis, but is often accompanied by a large proportion of by-products, especially for glycans containing sialic acids (Sia). Unlike hydroxyl groups of glycans, which are converted to stable methyl ethers by permethylation, the carboxylic acids on Sia are converted to methyl esters, which are easily reversible to carboxylate under alkaline conditions. To overcome this problem, we used linkage-specific alkylamidation to protect Sia prior to the permethylation. This method not only decreased the levels of by-products, but also enabled us to distinguish isomers of α2,3- and α2,6-Sia while simultaneously determining other glycosidic linkages.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Ácidos Siálicos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article