Rational Design of a DNA-Scaffolded High-Affinity Binder for Langerin.
Angew Chem Int Ed Engl
; 59(47): 21016-21022, 2020 11 16.
Article
em En
| MEDLINE
| ID: mdl-32749019
ABSTRACT
Binders of langerin could target vaccines to Langerhans cells for improved therapeutic effect. Since langerin has low affinity for monovalent glycan ligands, highly multivalent presentation has previously been key for targeting. Aiming to reduce the amount of ligand required, we rationally designed molecularly defined high-affinity binders based on the precise display of glycomimetic ligands (Glc2NTs) on DNA-PNA scaffolds. Rather than mimicking langerin's homotrimeric structure with a C3-symmetric scaffold, we developed readily accessible, easy-to-design bivalent binders. The method considers the requirements for bridging sugar binding sites and statistical rebinding as a means to both strengthen the interactions at single binding sites and amplify the avidity enhancement provided by chelation. This gave a 1150-fold net improvement over the affinity of the free ligand and provided a nanomolar binder (IC50 =300â
nM) for specific internalization by langerin-expressing cells.
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Base de dados:
MEDLINE
Assunto principal:
DNA
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Antígenos CD
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Lectinas Tipo C
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Lectinas de Ligação a Manose
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article