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Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens.
Mboneni, Tondani A; Eales, Owen O; Mosina, Ntsoaki L; Fourie, P Bernard.
Afiliação
  • Mboneni TA; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.
  • Eales OO; Department of Family Medicine, University of Pretoria, Pretoria, South Africa.
  • Mosina NL; Department of Genetics, University of Pretoria, Pretoria, South Africa.
  • Fourie PB; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.
J Med Microbiol ; 69(9): 1179-1182, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32755530
ABSTRACT
Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens (n=61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular transport medium (PS-MTM). Mycobacterium tuberculosis was grown in culture from 13 (21.3 %) and Xpert reported 15 (24.2 %) positive, of which 10 concordant. RT-PCR of PS-MTM samples showed enhanced sensitivity; three positives were missed by Xpert, five by culture and three more detected for a total of 21 positives (34.4 %).
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tuberculose Pulmonar / Tosse / Mycobacterium tuberculosis Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tuberculose Pulmonar / Tosse / Mycobacterium tuberculosis Idioma: En Ano de publicação: 2020 Tipo de documento: Article