Use of Engineered Nanoparticles (ENPs) for the Study of High-Affinity IgE FcεRI Receptor Engagement and Rat Basophilic Leukemia (RBL) Cell Degranulation.
Methods Mol Biol
; 2163: 171-180, 2020.
Article
em En
| MEDLINE
| ID: mdl-32766975
ABSTRACT
Degranulation of mast cells and basophils occurs after the cross-linking of FcεRI receptor-bound IgE by multivalent allergens, resulting in the release of a range of de novo synthesized and preformed mediators of the allergic response. ß-Hexosaminidase release is usually measured as a simple readout for degranulation. Furthermore, the rat basophilic leukemia (RBL)-2H3 cell line is commonly used for measuring degranulation, monitoring ß-hexosaminidase release. Here, we describe surface-engineered and modified nanoparticles with specific ligands in order to study the signaling and cellular responses of the RBL-2H3 cell line.
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Base de dados:
MEDLINE
Assunto principal:
Basófilos
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Imunoglobulina E
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Teste de Degranulação de Basófilos
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Degranulação Celular
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Receptores de IgE
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Nanopartículas
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article