The In Situ Structure of Parkinson's Disease-Linked LRRK2.
Cell
; 182(6): 1508-1518.e16, 2020 09 17.
Article
em En
| MEDLINE
| ID: mdl-32783917
ABSTRACT
Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent cause of familial Parkinson's disease. LRRK2 is a multi-domain protein containing a kinase and GTPase. Using correlative light and electron microscopy, in situ cryo-electron tomography, and subtomogram analysis, we reveal a 14-Å structure of LRRK2 bearing a pathogenic mutation that oligomerizes as a right-handed double helix around microtubules, which are left-handed. Using integrative modeling, we determine the architecture of LRRK2, showing that the GTPase and kinase are in close proximity, with the GTPase closer to the microtubule surface, whereas the kinase is exposed to the cytoplasm. We identify two oligomerization interfaces mediated by non-catalytic domains. Mutation of one of these abolishes LRRK2 microtubule-association. Our work demonstrates the power of cryo-electron tomography to generate models of previously unsolved structures in their cellular environment.
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Base de dados:
MEDLINE
Assunto principal:
Doença de Parkinson
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Microscopia Crioeletrônica
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Tomografia com Microscopia Eletrônica
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Serina-Treonina Proteína Quinase-2 com Repetições Ricas em Leucina
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Microtúbulos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article