Identification of DNA response elements regulating expression of CCAAT/enhancer-binding protein (C/EBP) ß and δ and MAP kinase-interacting kinases during early adipogenesis.
Adipocyte
; 9(1): 427-442, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-32787498
ABSTRACT
Given the high and increasing prevalence of obesity and associated disorders, such as type-2 diabetes, it is important to understand the mechanisms that regulate lipid storage and the differentiation of fat cells, a process termed adipogenesis. Using the well-established mouse 3T3-L1 in vitro model of adipogenesis, we refine how the induction of two key adipogenic transcription factors, CCAAT/enhancer-binding proteins (C/EBPs) ß and δ are regulated during early adipogenesis. We identify, in the gene promoters of Cebpb and Cebpd, the DNA response elements responsible for binding transcription factors that are activated by cAMP or glucocorticoids. We also show that mitogen-activated protein kinase (MAPK)-interacting kinase 2 (MNK2; Mknk2), which plays a distinct role in diet-induced obesity, is induced during early adipogenesis and identify the functional DNA response elements responsible for regulating its expression. Mknk2 expression is maintained in differentiated 3T3-L1 adipocytes and is expressed at high levels across a range of mouse adipose tissue depots. Together, these new insights help to clarify the transcriptional programme of early adipogenesis and identify Mknk2 as one of potentially many genes up-regulated during adipogenesis.
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Base de dados:
MEDLINE
Assunto principal:
Elementos de Resposta
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Quinases de Proteína Quinase Ativadas por Mitógeno
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Proteína beta Intensificadora de Ligação a CCAAT
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Proteína delta de Ligação ao Facilitador CCAAT
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Adipogenia
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article