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Sex matters: XIST and DDX3Y gene expression as a tool to determine fetal sex in human first trimester placenta.
Hoch, Denise; Novakovic, Boris; Cvitic, Silvija; Saffery, Richard; Desoye, Gernot; Majali-Martinez, Alejandro.
Afiliação
  • Hoch D; Department of Obstetrics and Gynecology, Medical University of Graz, Graz, 8036, Austria. Electronic address: denise.hoch@medunigraz.at.
  • Novakovic B; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, 3052, Australia. Electronic address: boris.novakovic@mcri.edu.au.
  • Cvitic S; Department of Obstetrics and Gynecology, Medical University of Graz, Graz, 8036, Austria; Department of Paediatrics and Adolescent Medicine, Research Unit of Analytical Mass Spectrometry, Cell Biology and Biochemistry of Inborn Errors of Metabolism, Medical University of Graz, Graz, 8036, Austria. E
  • Saffery R; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, 3052, Australia. Electronic address: richard.saffery@mcri.edu.au.
  • Desoye G; Department of Obstetrics and Gynecology, Medical University of Graz, Graz, 8036, Austria. Electronic address: gernot.desoye@medunigraz.at.
  • Majali-Martinez A; Department of Obstetrics and Gynecology, Medical University of Graz, Graz, 8036, Austria. Electronic address: a.majali-martinez@medunigraz.at.
Placenta ; 97: 68-70, 2020 08.
Article em En | MEDLINE | ID: mdl-32792067
ABSTRACT
Fetal sex influences placental function as well as maternal and fetal health, being an important factor to consider in pregnancy studies. However, fetal sex determination in the first trimester of pregnancy still faces some technical limitations. Here we describe an RT-qPCR technique to determine fetal sex based on X-inactive specific transcript (XIST) and DEAD-Box helicase 3 Y-linked (DDX3Y) gene expression. This method is straightforward, reliable, fast and applicable on both, placental tissue and primary cells.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Placenta / Primeiro Trimestre da Gravidez / Análise para Determinação do Sexo / Expressão Gênica / Antígenos de Histocompatibilidade Menor / RNA Helicases DEAD-box / RNA Longo não Codificante Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Placenta / Primeiro Trimestre da Gravidez / Análise para Determinação do Sexo / Expressão Gênica / Antígenos de Histocompatibilidade Menor / RNA Helicases DEAD-box / RNA Longo não Codificante Idioma: En Ano de publicação: 2020 Tipo de documento: Article