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Mycoplasma hyopneumoniae J elicits an antioxidant response and decreases the expression of ciliary genes in infected swine epithelial cells.
Mucha, Scheila G; Ferrarini, Mariana G; Moraga, Carol; Di Genova, Alex; Guyon, Laurent; Tardy, Florence; Rome, Sophie; Sagot, Marie-France; Zaha, Arnaldo.
Afiliação
  • Mucha SG; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Ferrarini MG; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Moraga C; Université de Lyon, Université Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive UMR 5558, 69622, Villeurbanne, France.
  • Di Genova A; Université de Lyon, Université Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive UMR 5558, 69622, Villeurbanne, France.
  • Guyon L; ERABLE, Inria, Lyon, France.
  • Tardy F; Université de Lyon, Université Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive UMR 5558, 69622, Villeurbanne, France.
  • Rome S; ERABLE, Inria, Lyon, France.
  • Sagot MF; Université Grenoble Alpes, CEA, INSERM, IRIG, Biology of Cancer and Infection UMR_S 1036, 38000, Grenoble, France.
  • Zaha A; Université de Lyon, Anses, Laboratoire de Lyon, UMR Mycoplasmoses des Ruminants, Lyon, France.
Sci Rep ; 10(1): 13707, 2020 08 13.
Article em En | MEDLINE | ID: mdl-32792522
ABSTRACT
Mycoplasma hyopneumoniae is the most costly pathogen for swine production. Although several studies have focused on the host-bacterium association, little is known about the changes in gene expression of swine cells upon infection. To improve our understanding of this interaction, we infected swine epithelial NPTr cells with M. hyopneumoniae strain J to identify differentially expressed mRNAs and miRNAs. The levels of 1,268 genes and 170 miRNAs were significantly modified post-infection. Up-regulated mRNAs were enriched in genes related to redox homeostasis and antioxidant defense, known to be regulated by the transcription factor NRF2 in related species. Down-regulated mRNAs were enriched in genes associated with cytoskeleton and ciliary functions. Bioinformatic analyses suggested a correlation between changes in miRNA and mRNA levels, since we detected down-regulation of miRNAs predicted to target antioxidant genes and up-regulation of miRNAs targeting ciliary and cytoskeleton genes. Interestingly, most down-regulated miRNAs were detected in exosome-like vesicles suggesting that M. hyopneumoniae infection induced a modification of the composition of NPTr-released vesicles. Taken together, our data indicate that M. hyopneumoniae elicits an antioxidant response induced by NRF2 in infected cells. In addition, we propose that ciliostasis caused by this pathogen is partially explained by the down-regulation of ciliary genes.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Cílios / Mycoplasma hyopneumoniae / Pneumonia Suína Micoplasmática / Células Epiteliais / Antioxidantes Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Cílios / Mycoplasma hyopneumoniae / Pneumonia Suína Micoplasmática / Células Epiteliais / Antioxidantes Idioma: En Ano de publicação: 2020 Tipo de documento: Article