Beta adrenergic receptor activation inhibits oral cancer migration and invasiveness.
Arch Oral Biol
; 118: 104865, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-32801034
ABSTRACT
OBJECTIVE:
The aim of this study was to verify ß2-AR expression in oral squamous cell carcinoma cell lines (SCC-9 and SCC-25), and to investigate the role of this receptor in migration and invasion of these neoplastic cells.DESIGN:
SCC-9 and SCC-25 cells were investigated for gene and protein expression of ß2-AR. Cell migration and invasion were analyzed by wound healing assay and transwell invasion camera system. Different concentrations (0.1, 1 and 10⯵M) of norepinephrine were used to stimulate, and 1⯵M propranolol was used to block the beta-adrenergic receptors on cancer cells. Differences in median values of SCC-9 and SCC-25 and ß2-AR protein expression were analyzed by Friedman test and in case of significant differences; pairwise comparisons were performed using Bonferroni correction.RESULTS:
The results showed that the ß2-AR gene and protein expression were observed in both oral cancer cell lines. The concentration of 10⯵M of norepinephrine significantly inhibited (pâ¯≤â¯0.05) migration of SCC-9 and SCC-25 cell lines. Furthermore, there was a significant reduction (pâ¯≤â¯0.05) in the effect of norepinephrine on cell migration when the ß2-AR was inhibited by propranolol. The blockade by propranolol showed a tendency to reverse the effect of norepinephrine on the invasiveness of SCC-9 and SCC-25.CONCLUSIONS:
The use of beta-adrenergic receptor agonists could become an adjuvant therapeutic target in the treatment of this malignancy.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Bucais
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Carcinoma de Células Escamosas
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Receptores Adrenérgicos beta
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Invasividade Neoplásica
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article