Mouse Model for Efficient Simultaneous Targeting of Glycolysis, Glutaminolysis, and De Novo Synthesis of Fatty Acids in Colon Cancer.
Methods Mol Biol
; 2174: 45-69, 2021.
Article
em En
| MEDLINE
| ID: mdl-32813244
ABSTRACT
Colon cancer is a highly anabolic entity with upregulation of glycolysis, glutaminolysis, and de novo synthesis of fatty acids, which also induces a hypercatabolic state in the patient. The blockade of either cancer anabolism or host catabolism has been previously proven to be a successful anticancer experimental treatment. However, it is still unclear whether the simultaneous blockade of both metabolic counterparts can limit malignant survival and the energetic consequences of such an approach. In this chapter, by using the CT26.WT murine colon adenocarcinoma cell line as a model of study, we provide a method to simultaneously perform a pharmacological blockade of tumor anabolism and host catabolism, as a feasible therapeutic approach to treat cancer, and to limit its energetic supply.
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Base de dados:
MEDLINE
Assunto principal:
Adenocarcinoma
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Neoplasias do Colo
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Ácidos Graxos
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Glutamina
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Antineoplásicos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article