Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase.
Carbohydr Polym
; 247: 116714, 2020 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-32829841
ABSTRACT
Enzymatic transformation of xylans into renewable fuels and value-added products is mediated by xylanases. Here we describe the biochemical and X-ray structural characterization of Thermobacillus composti GH10 xylanase (TcXyn10A) at 2.1 Å resolution aiming to unravel details of its recognition of glucurono- and arabinoxylan at a molecular level. TcXyn10A improves the efficiency of pretreated lignocellulosic biomass hydrolysis by a commercial enzyme cocktail causing a 15.35 % increase in xylose release and 4.38 % glucose release after 24 h of reaction. The enzyme releases predominantly xylobiose and xylotriose, as well as MeGlcA3 × 3 (from beechwood glucuronoxylan) and a range of decorated xylooligosaccharides (XOS) from rye arabinoxylan, with Ara2 × 2 being the major product. The enzyme liberates XOS with the yields of 29.09 % for beechwood glucuronoxylan and 16.98 % for rye arabinoxylan. Finally, TcXyn10A has a high thermal stability, halotolerance, and resistance to ethanol, biochemical properties that can be desirable for a number of industrial applications.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Xilanos
/
Endo-1,4-beta-Xilanases
/
Bacillales
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article