Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures.
Methods Mol Biol
; 2203: 119-134, 2020.
Article
em En
| MEDLINE
| ID: mdl-32833209
ABSTRACT
Well-differentiated primary airway epithelial cell (AEC) cultures have been widely used for the characterization of several human respiratory viruses including coronaviruses. In recent years, there has been an increase in interest toward animal AEC cultures and their application to characterize veterinary viruses with zoonotic potential, as well as studying host-pathogen interactions in animal reservoir host species. In this chapter, we provide a revised and improved protocol for the isolation and establishment of well-differentiated AEC cultures from diverse mammalian species and the use of the cultures for the characterization of veterinary coronavirus. We also describe immunohistochemistry protocols with validated antibodies for the visualization and identification of viral cell tropism in well-differentiated AEC cultures from human, swine, bovine, and feline origin.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Traqueia
/
Brônquios
/
Coronavirus
/
Células Epiteliais
/
Cultura Primária de Células
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article