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Human skeletal muscle metabolic responses to 6 days of high-fat overfeeding are associated with dietary n-3PUFA content and muscle oxidative capacity.
Wardle, Sophie L; Macnaughton, Lindsay S; McGlory, Chris; Witard, Oliver C; Dick, James R; Whitfield, Philip D; Ferrando, Arny A; Wolfe, Robert R; Kim, Il-Young; Hamilton, D Lee; Moran, Colin N; Tipton, Kevin D; Galloway, Stuart D R.
Afiliação
  • Wardle SL; Physiology, Exercise and Nutrition Research Group, University of Stirling, Stirling, UK.
  • Macnaughton LS; Army Health and Performance Research, Army Headquarters, Andover, UK.
  • McGlory C; Physiology, Exercise and Nutrition Research Group, University of Stirling, Stirling, UK.
  • Witard OC; Sportscotland Institute of Sport, Stirling, UK.
  • Dick JR; Physiology, Exercise and Nutrition Research Group, University of Stirling, Stirling, UK.
  • Whitfield PD; Queens University, Kingston, Ontario, Canada.
  • Ferrando AA; Physiology, Exercise and Nutrition Research Group, University of Stirling, Stirling, UK.
  • Wolfe RR; Centre for Human and Applied Physiological Sciences, King's College London, London, UK.
  • Kim IY; Nutrition Group, Institute of Aquaculture, University of Stirling, Stirling, UK.
  • Hamilton DL; Lipidomics Research Facility, Division of Biomedical Sciences, University of the Highlands and Islands, Inverness, UK.
  • Moran CN; Department of Geriatrics, Center for Translational Research in Aging and Longevity, Donald W. Reynolds Institute on Aging, Little Rock, AR, USA.
  • Tipton KD; Department of Geriatrics, Center for Translational Research in Aging and Longevity, Donald W. Reynolds Institute on Aging, Little Rock, AR, USA.
  • Galloway SDR; Department of Geriatrics, Center for Translational Research in Aging and Longevity, Donald W. Reynolds Institute on Aging, Little Rock, AR, USA.
Physiol Rep ; 8(16): e14529, 2020 08.
Article em En | MEDLINE | ID: mdl-32845565
ABSTRACT
Understanding human physiological responses to high-fat energy excess (HFEE) may help combat the development of metabolic disease. We aimed to investigate the impact of manipulating the n-3PUFA content of HFEE diets on whole-body and skeletal muscle markers of insulin sensitivity. Twenty healthy males were overfed (150% energy, 60% fat, 25% carbohydrate, 15% protein) for 6 d. One group (n = 10) received 10% of fat intake as n-3PUFA rich fish oil (HF-FO), and the other group consumed a mix of fats (HF-C). Oral glucose tolerance tests with stable isotope tracer infusions were conducted before, and following, HFEE, with muscle biopsies obtained in basal and insulin-stimulated states for measurement of membrane phospholipids, ceramides, mitochondrial enzyme activities, and PKB and AMPKα2 activity. Insulin sensitivity and glucose disposal did not change following HFEE, irrespective of group. Skeletal muscle ceramide content increased following HFEE (8.5 ± 1.2 to 12.1 ± 1.7 nmol/mg, p = .03), irrespective of group. No change in mitochondrial enzyme activity was observed following HFEE, but citrate synthase activity was inversely associated with the increase in the ceramide content (r=-0.52, p = .048). A time by group interaction was observed for PKB activity (p = .003), with increased activity following HFEE in HF-C (4.5 ± 13.0mU/mg) and decreased activity in HF-FO (-10.1 ± 20.7 mU/mg) following HFEE. Basal AMPKα2 activity increased in HF-FO (4.1 ± 0.6 to 5.3 ± 0.7mU/mg, p = .049), but did not change in HF-C (4.6 ± 0.7 to 3.8 ± 0.9mU/mg) following HFEE. We conclude that early skeletal muscle signaling responses to HFEE appear to be modified by dietary n-3PUFA content, but the potential impact on future development of metabolic disease needs exploring.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Hiperfagia / Ácidos Graxos Ômega-3 / Músculo Esquelético / Dieta Hiperlipídica Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Hiperfagia / Ácidos Graxos Ômega-3 / Músculo Esquelético / Dieta Hiperlipídica Idioma: En Ano de publicação: 2020 Tipo de documento: Article