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The investigation of calpain in human placenta with fetal growth restriction.
Goto, Shinobu; Ozaki, Yasuhiko; Ozawa, Fumiko; Mizutani, Eita; Kitaori, Tamao; Suzumori, Nobuhiro; Blomgren, Klas; Furuno, Tadahide; Sugiura-Ogasawara, Mayumi.
Afiliação
  • Goto S; Department of Obstetrics and Gynecology, Nagoya City University, Graduate School of Medical Sciences, Nagoya, Japan.
  • Ozaki Y; Division of Clinical and Molecular Genetics, Nagoya City University, Graduate School of Medical Sciences, Nagoya, Japan.
  • Ozawa F; Research Center for Recurrent Pregnancy Loss, Nagoya City University, Graduate School of Medical Sciences, Nagoya, Japan.
  • Mizutani E; Department of Obstetrics and Gynecology, Nagoya City University, Graduate School of Medical Sciences, Nagoya, Japan.
  • Kitaori T; Research Center for Recurrent Pregnancy Loss, Nagoya City University, Graduate School of Medical Sciences, Nagoya, Japan.
  • Suzumori N; The Education and Research Center for Advanced Medicine, Nagoya City University, Graduate School of Medical Sciences, Nagoya, Japan.
  • Blomgren K; Research Center for Recurrent Pregnancy Loss, Nagoya City University, Graduate School of Medical Sciences, Nagoya, Japan.
  • Furuno T; Daiya Building Lady's Clinic, Nagoya, Japan.
  • Sugiura-Ogasawara M; Department of Obstetrics and Gynecology, Nagoya City University, Graduate School of Medical Sciences, Nagoya, Japan.
Am J Reprod Immunol ; 85(1): e13325, 2021 01.
Article em En | MEDLINE | ID: mdl-32852077
ABSTRACT

PROBLEM:

The mechanism of fetal growth restriction (FGR) is not fully understood. In this study, we explored the contribution of the calpain-calpastatin system and the activated states of calpains in human FGR placenta. METHOD OF STUDY The placentas were collected from patients of FGR (n = 17) and controls (n = 23) at elective cesarean sections in Nagoya City University Hospital and used for experiments upon informed consent. The existence and the expression of calpains and calpastatin in human placenta were compared between FGR and controls using immunohistochemistry, SDS-PAGE, and Western blotting.

RESULTS:

Staining of calpains (pre-, post-µ-calpain, pre-, post-m-calpain, and calpain-6) and calpastatin was observed in cytoplasm of trophoblast cells, both in FGR and control placenta. Pre-µ-calpain was located in the cytoplasm, and post-µ-calpain was located mainly in proximity to the cytoplasmic membrane. The expression of pre-µ-calpain was significantly higher (P < .001) and calpain-6 was significantly lower (P = .01) in FGR placentas. The inactive µ-calpain (80 kDa) was significantly elevated (P < .01), and active µ-calpain (76 kDa) was significantly decreased (P = .01) in FGR placentas.

CONCLUSION:

The results demonstrate that activation of µ-calpain is suppressed in FGR placentas and that calpain-6 in human placenta is involved in the pathology of FGR.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Placenta / Calpaína / Retardo do Crescimento Fetal Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Placenta / Calpaína / Retardo do Crescimento Fetal Idioma: En Ano de publicação: 2021 Tipo de documento: Article