Tag-Free, Temperature Dependent Infrared Spectra of the GFP Chromophore: Revisiting the Question of Isomerism.
J Phys Chem A
; 124(38): 7827-7831, 2020 Sep 24.
Article
em En
| MEDLINE
| ID: mdl-32866387
ABSTRACT
We report infrared spectra of a model chromophore of green fluorescent protein, prepared in an ion trap at temperatures ranging from 30 K to room temperature. We compare the changes in the infrared spectrum with predicted infrared spectra for the Z and E isomers of this molecule, and we confirm that the molecule exists as the Z isomer at low temperatures. We revisit the question whether or not it can thermally isomerize in the temperature range of this experiment, and we find no evidence for isomerization.
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Base de dados:
MEDLINE
Assunto principal:
Temperatura
/
Imidazolinas
/
Proteínas de Fluorescência Verde
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article