Records of RNA locations in living yeast revealed through covalent marks.
Proc Natl Acad Sci U S A
; 117(38): 23539-23547, 2020 09 22.
Article
em En
| MEDLINE
| ID: mdl-32907940
ABSTRACT
RNA movements and localization pervade biology, from embryonic development to disease. To identify RNAs at specific locations, we developed a strategy in which a uridine-adding enzyme is anchored to subcellular sites, where it directly marks RNAs with 3' terminal uridines. This localized RNA recording approach yields a record of RNA locations, and is validated through identification of RNAs localized selectively to the endoplasmic reticulum (ER) or mitochondria. We identify a broad dual localization pattern conserved from yeast to human cells, in which the same battery of mRNAs encounter both ER and mitochondria in both species, and include an mRNA encoding a key stress sensor. Subunits of many multiprotein complexes localize to both the ER and mitochondria, suggesting coordinated assembly. Noncoding RNAs in the course of RNA surveillance and processing encounter both organelles. By providing a record of RNA locations over time, the approach complements those that capture snapshots of instantaneous positions.
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MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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RNA Fúngico
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Proteínas de Saccharomyces cerevisiae
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article