Control of the growth of Alicyclobacillus acidoterrestris in industrialized orange juice using rosemary essential oil and nisin.
Lett Appl Microbiol
; 72(1): 41-52, 2021 Jan.
Article
em En
| MEDLINE
| ID: mdl-32910828
ABSTRACT
The use of rosemary essential oil (RO) and its combination with nisin (RO+N) in preventing the multiplication of Alicyclobacillus acidoterrestris in orange juice was evaluated. The minimum inhibitory and bactericidal concentrations (MIC and MBC) for RO were both 125 µg ml-1 while RO+N displayed a synergistic effect. The use of RO and RO+N at concentrations of 1, 4 and 8× MIC in orange juice for 96 h was evaluated in terms of their sporicidal effectiveness. With regard to the action against A. acidoterrestris spores, RO at 8× MIC was sporostatic, whereas RO+N at 1× MIC was sporicidal. Morphological changes in the structure of the micro-organism after treatment were also observed by microscopy. Furthermore, flow cytometric analysis showed that most cells were damaged or killed after treatment. In general, the antioxidant activity after addition of RO+N decreased with time. The results demonstrate that using the combination of RO and nisin can prevent the A. acidoterrestris growth in orange juice.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Óleos Voláteis
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Rosmarinus
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Alicyclobacillus
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Sucos de Frutas e Vegetais
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Antibacterianos
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Nisina
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article