Characterization of inthomycin biosynthetic gene cluster revealing new insights into carboxamide formation.
Chin J Nat Med
; 18(9): 677-683, 2020 Sep.
Article
em En
| MEDLINE
| ID: mdl-32928511
ABSTRACT
Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B (1) and its two new analogues 2 and 3 were isolated from the crude extract of Streptomyces pactum L8. Identification of the gene cluster for inthomycin biosynthesis as well as the 15N-labeled glycine incorporation into inthomycins are described. Combined with the gene deletion of the rare P450 domain in the NRPS module, a formation mechanism of carboxamide moiety in inthomycins was proposed via an oxidative release of the assembly chain assisted by the P450 domain.
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Graxos Insaturados
/
Antibacterianos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article