Adding to the CASeload: unwarranted p53 signaling induced by Cas9.
Mol Cell Oncol
; 7(5): 1789419, 2020.
Article
em En
| MEDLINE
| ID: mdl-32944644
ABSTRACT
We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines. For a subset of cell lines with a wild-type tumor protein TP53 (best known as p53), we detected p53 pathway activation, DNA damage accumulation and emerging p53-inactivating mutations following Cas9 introduction. We discuss the potential implications of our findings in basic and translational research.
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MEDLINE
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En
Ano de publicação:
2020
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Article