Characterisation of IL-15 and IL-2Rß in grass carp: IL-15 upregulates cytokines and transcription factors of type 1 immune response and NK cell activation.
Fish Shellfish Immunol
; 107(Pt A): 104-117, 2020 Dec.
Article
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| MEDLINE
| ID: mdl-32971272
ABSTRACT
Interleukin (IL) -15 belongs to the common cytokine receptor γ chain (γC) family and has diverse functions in regulating the development, proliferation and activation of NK and T cells. It activates a hetero-trimeric receptor complex consisting of IL-2Rα, IL-2Rß and a common γ chain (γC). In this study, the full-length cDNA sequences of IL-15 and IL-2Rß were identified in grass carp (Ctenopharyngodon idella, Ci) and their expression profiles analysed. The CiIL-15 and CiIL-2Rß were shown to be broadly expressed in tissues, with the highest levels detected in the spleen. Moreover, the CiIL-15 and CiIL-2Rß were modulated in primary head kidney leucocytes (HKLs) and splenocytes by immunostimulants and cytokines, and in the head kidney and spleen of fish after infection of Flavobacterium columnare and grass carp reovirus. The bioactivity of bacteria derived recombinant CiIL-15 protein was evaluated in the primary leucocytes. The CiIL-15 was shown to induce signature genes of type 1 immune response (IFN-γ and T-bet) and NK cell activation (perforin and Eomesa), whilst exhibiting inhibitory effects on the genes involved in the type 2 immune response (IL-4/13, IL-10 and Gata3). Our data suggest that IL-15 is a key regulator in promoting the type 1 immune response and NK cell activation in fish.
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Base de dados:
MEDLINE
Assunto principal:
Perciformes
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Regulação da Expressão Gênica
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Proteínas de Peixes
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Doenças dos Peixes
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Imunidade Inata
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article