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Dual COX and 5-LOX inhibition by clerodane diterpenes from seeds of Polyalthia longifolia (Sonn.) Thwaites.
Nguyen, Ha Thi; Vu, Thien-Y; Chandi, Vishala; Polimati, Haritha; Tatipamula, Vinay Bharadwaj.
Afiliação
  • Nguyen HT; Institute of Research and Development, Duy Tan University, Da Nang, 550000, Vietnam.
  • Vu TY; Faculty of Medicine, Duy Tan University, Da Nang, 550000, Vietnam.
  • Chandi V; Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, 700000, Vietnam.
  • Polimati H; Pharmacology Department, AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, 530003, India.
  • Tatipamula VB; Pharmacology Department, AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, 530003, India.
Sci Rep ; 10(1): 15965, 2020 09 29.
Article em En | MEDLINE | ID: mdl-32994508
Natural metabolites with their specific bioactivities are being considered as a potential source of materials for pharmacological studies. In this study, we successfully isolated and identified five known clerodane diterpenes, namely 16-oxo-cleroda-3,13(14)E-dien-15-oic acid (1), 16-hydroxy-cleroda-3,13-dien-15-oic acid (2), 16-hydroxy-cleroda-4(18),13-dien-16,15-olide (3), 3α,16α-dihydroxy-cleroda-4(18),13(14)Z-dien-15,16-olide (4), and 16α-hydroxy-cleroda-3,13(14)Z-dien-15,16-olide (5) from the methanolic extract of seeds of Polyalthia longifolia. Initially, all the isolated metabolites were investigated for COX-1, COX-2, and 5-LOX inhibitory activities using the standard inhibitory kits. Of which, compounds 3, 4, and 5 exhibited to be potent COX-1, COX-2, and 5-LOX inhibitors with the IC50 values similar or lower to those of the reference drugs. To understand the underlying mechanism, these compounds were subjected to molecular docking on COX-1, COX-2, and 5-LOX proteins. Interestingly, the in silico study results were in high accordance with in vitro studies where compounds 3, 4, and 5 hits assumed interactions and binding pattern comparable to that of reference drugs (indomethacin and diclofenac), as a co-crystallized ligand explaining their remarkable dual (COX/LOX) inhibitor actions. Taken together, our findings demonstrated that compounds 3, 4, and 5 functioned as dual inhibitors of COX/5-LOX and can contribute to the development of novel, more effective anti-inflammatory drugs with minimal side-effects.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Araquidonato 5-Lipoxigenase / Polyalthia / Diterpenos Clerodânicos / Ciclo-Oxigenase 1 / Ciclo-Oxigenase 2 Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Araquidonato 5-Lipoxigenase / Polyalthia / Diterpenos Clerodânicos / Ciclo-Oxigenase 1 / Ciclo-Oxigenase 2 Idioma: En Ano de publicação: 2020 Tipo de documento: Article