Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize.
Genome Biol
; 21(1): 257, 2020 10 06.
Article
em En
| MEDLINE
| ID: mdl-33023639
ABSTRACT
Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Acetolactato Sintase
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Mutagênese Sítio-Dirigida
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RNA Guia de Cinetoplastídeos
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Zea mays
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Edição de Genes
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article