Characterization and functional analysis of a galectin-related protein B from Nile tilapia involved in the immune response to bacterial infection.
J Fish Dis
; 44(2): 171-180, 2021 Feb.
Article
em En
| MEDLINE
| ID: mdl-33040388
ABSTRACT
Galectin-related protein is a kind of lectin without canonical activity that regulates cell adhesion and cell growth. In this study, a novel galectin-related protein B (OnGRPB) was identified from Nile tilapia (Oreochromis niloticus). The open reading frame of OnGRPB was 438 bp and encoded a peptide of 145 amino acids. The deduced protein sequence of OnGRPB possessed a conserved carbohydrate recognition domain (CRD) with partial sugar binding sites (N-R, V-N and W-E) and shared high identities with other fish GRPB proteins. The qRT-PCR analysis found that OnGRPB was widely distributed in various tissues and monocyte/macrophages (Mo/MΦ) of healthy tilapia. After Streptococcus agalactiae infection, OnGRPB transcripts were significantly up-regulated in liver, spleen, head kidney and Mo/MΦ. The recombinant OnGRPB protein (rOnGRPB) had the binding activity and agglutination ability to bacteria. Also, rOnGRPB could modulate antibacterial activity and inflammatory factor expression of Mo/MΦ. These data collectively indicate that OnGRPB plays roles in the immune response of Nile tilapia against bacterial infection.
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Base de dados:
MEDLINE
Assunto principal:
Infecções Estreptocócicas
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Ciclídeos
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Galectinas
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Doenças dos Peixes
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article