A Small Molecule Fluorogenic Probe for the Detection of Sphingosine in Living Cells.
J Am Chem Soc
; 142(42): 17887-17891, 2020 10 21.
Article
em En
| MEDLINE
| ID: mdl-33044062
ABSTRACT
The single-chained sphingolipid sphingosine is an essential structural lipid and signaling molecule. Abnormal sphingosine metabolism is observed in several diseases, including cancer, diabetes, and Alzheimer's. Despite its biological importance, there is a lack of tools for detecting sphingosine in living cells. This is likely due to the broader challenge of developing highly selective and live-cell compatible affinity probes for hydrophobic lipid species. In this work, we have developed a small molecule fluorescent turn-on probe for labeling sphingosine in living cells. We demonstrate that this probe exhibits a dose-dependent response to sphingosine and is able to detect endogenous pools of sphingosine. Using our probe, we successfully detected sphingosine accumulation in cells from patients with Niemann-Pick type C1 (NPC1), a lipid transport disorder in which increased sphingosine mediates disease progression. This work provides a simple and accessible method for the detection of sphingosine and should facilitate study of this critical signaling lipid in biology and disease.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Esfingosina
/
Aldeídos
/
Bibliotecas de Moléculas Pequenas
/
Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article