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An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli.
Zambaldo, Claudio; Koh, Minseob; Nasertorabi, Fariborz; Han, Gye Won; Chatterjee, Abhishek; Stevens, Raymond C; Schultz, Peter G.
Afiliação
  • Zambaldo C; Department of Chemistry and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, United States.
  • Koh M; Department of Chemistry and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, United States.
  • Nasertorabi F; Departments of Biological Sciences and Chemistry, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, CA 90089, United States.
  • Han GW; Departments of Biological Sciences and Chemistry, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, CA 90089, United States.
  • Chatterjee A; Department of Chemistry, Boston College, 2609 Beacon Street, 246B Merkert Chemistry Center, Chestnut Hill, MA 02467, United States. Electronic address: abhishek.chatterjee@bc.edu.
  • Stevens RC; Departments of Biological Sciences and Chemistry, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, CA 90089, United States. Electronic address: stevens@usc.edu.
  • Schultz PG; Department of Chemistry and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, United States. Electronic address: schultz@scripps.edu.
Bioorg Med Chem ; 28(20): 115662, 2020 10 15.
Article em En | MEDLINE | ID: mdl-33069069
ABSTRACT
We report the development of the orthogonal amber-suppressor pair Archaeoglobus fulgidus seryl-tRNA (Af-tRNASer)/Methanosarcina mazei seryl-tRNA synthetase (MmSerRS) in Escherichia coli. Furthermore, the crystal structure of MmSerRS was solved at 1.45 Å resolution, which should enable structure-guided engineering of its active site to genetically encode small, polar noncanonical amino acids (ncAAs).
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Serina-tRNA Ligase / RNA de Transferência / Escherichia coli / Aminoácidos Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Serina-tRNA Ligase / RNA de Transferência / Escherichia coli / Aminoácidos Idioma: En Ano de publicação: 2020 Tipo de documento: Article