Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation.
Nat Commun
; 11(1): 5604, 2020 11 05.
Article
em En
| MEDLINE
| ID: mdl-33154375
ABSTRACT
Many animal embryos pull and close an epithelial sheet around the ellipsoidal egg surface during a gastrulation process known as epiboly. The ovoidal geometry dictates that the epithelial sheet first expands and subsequently compacts. Moreover, the spreading epithelium is mechanically stressed and this stress needs to be released. Here we show that during extraembryonic tissue (serosa) epiboly in the insect Tribolium castaneum, the non-proliferative serosa becomes regionalized into a solid-like dorsal region with larger non-rearranging cells, and a more fluid-like ventral region surrounding the leading edge with smaller cells undergoing intercalations. Our results suggest that a heterogeneous actomyosin cable contributes to the fluidization of the leading edge by driving sequential eviction and intercalation of individual cells away from the serosa margin. Since this developmental solution utilized during epiboly resembles the mechanism of wound healing, we propose actomyosin cable-driven local tissue fluidization as a conserved morphogenetic module for closure of epithelial gaps.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Epitélio
/
Gastrulação
/
Insetos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article