Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase.
Biopolymers
; 112(1): e23407, 2021 Jan.
Article
em En
| MEDLINE
| ID: mdl-33156531
ABSTRACT
We present cognate base pair selectivity in template-dependent ligation by T4 DNA ligase using a hydrophobic unnatural base pair (UBP), Ds-Pa. T4 DNA ligase efficiently recognizes the Ds-Pa pairing at the conjugation position, and Ds excludes the noncognate pairings with the natural bases. Our results indicate that the hydrophobic base pairing is allowed in enzymatic ligation with higher cognate base-pair selectivity, relative to the hydrogen-bond interactions between pairing bases. The efficient ligation using Ds-Pa can be employed in recombinant DNA technology using genetic alphabet expansion, toward the creation of semi-synthetic organisms containing UBPs.
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Base de dados:
MEDLINE
Assunto principal:
DNA Ligases
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DNA
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Nucleotídeos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article