Expanding the Chemical Diversity of Genetically Encoded Libraries.
ACS Comb Sci
; 22(12): 712-733, 2020 12 14.
Article
em En
| MEDLINE
| ID: mdl-33167616
ABSTRACT
The power of ribosomes has increasingly been harnessed for the synthesis and selection of molecular libraries. Technologies, such as phage display, yeast display, and mRNA display, effectively couple genotype to phenotype for the molecular evolution of high affinity epitopes for many therapeutic targets. Genetic code expansion is central to the success of these technologies, allowing researchers to surpass the intrinsic capabilities of the ribosome and access new, genetically encoded materials for these selections. Here, we review techniques for the chemical expansion of genetically encoded libraries, their abilities and limits, and opportunities for further development. Importantly, we also discuss methods and metrics used to assess the efficiency of modification and library diversity with these new techniques.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribossomos
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RNA Mensageiro
/
Biblioteca de Peptídeos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article