Using Genetic Code Expansion for Protein Biochemical Studies.
Front Bioeng Biotechnol
; 8: 598577, 2020.
Article
em En
| MEDLINE
| ID: mdl-33195171
ABSTRACT
Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the unique properties of non-canonical amino acids, we can identify spatiotemporal-specific protein states within living cells. Insertion of more than one non-canonical amino acid allows for selective labeling that can aid in the identification of weak or transient protein-protein interactions. This review will discuss recent studies applying genetic code expansion for protein labeling and identifying protein-protein interactions and offer considerations for future work in expanding genetic code expansion methods.
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MEDLINE
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En
Ano de publicação:
2020
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Article