Flow-Cytometric Analysis and Purification of Airway Epithelial-Cell Subsets.
Am J Respir Cell Mol Biol
; 64(3): 308-317, 2021 03.
Article
em En
| MEDLINE
| ID: mdl-33196316
ABSTRACT
The human airway epithelium is essential in homeostasis, and epithelial dysfunction contributes to chronic airway disease. Development of flow-cytometric methods to characterize subsets of airway epithelial cells will enable further dissection of airway epithelial biology. Leveraging single-cell RNA-sequencing data in combination with known cell type-specific markers, we developed panels of antibodies to characterize and isolate the major airway epithelial subsets (basal, ciliated, and secretory cells) from human bronchial epithelial-cell cultures. We also identified molecularly distinct subpopulations of secretory cells and demonstrated cell subset-specific expression of low-abundance transcripts and microRNAs that are challenging to analyze with current single-cell RNA-sequencing methods. These new tools will be valuable for analyzing and separating airway epithelial subsets and interrogating airway epithelial biology.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sistema Respiratório
/
Separação Celular
/
Células Epiteliais
/
Citometria de Fluxo
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article