Structure of the O-Antigen and the Lipidâ
A from the Lipopolysaccharide of Fusobacterium nucleatum ATCC 51191.
Chembiochem
; 22(7): 1252-1260, 2021 04 06.
Article
em En
| MEDLINE
| ID: mdl-33197108
ABSTRACT
Fusobacterium nucleatum is a common member of the oral microbiota. However, this symbiont has been found to play an active role in disease development. As a Gram-negative bacterium, F. nucleatum has a protective outer membrane layer whose external leaflet is mainly composed of lipopolysaccharides (LPSs). LPSs play a crucial role in the interaction between bacteria and the host immune system. Here, we characterised the structure of the O-antigen and lipidâ
A from F. nucleatum ssp. animalis ATCC 51191 by using a combination of GC-MS, MALDI and NMR techniques. The results revealed a novel repeat of the O-antigen structure of the LPS, [â4)-ß-d-GlcpNAcA-(1â4)-ß-d-GlcpNAc3NAlaA-(1â3)-α-d-FucpNAc4NR-(1â], (R=acetylated 60 %), and a bis-phosphorylated hexa-acylated lipidâ
A moiety. Taken together these data showed that F. nucleatum ATCC 51191 has a distinct LPS which might differentially influence recognition by immune cells.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
/
Fusobacterium nucleatum
/
Antígenos O
/
Lipídeo A
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article