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HER2 gene assessment in liquid biopsy of gastric and esophagogastric junction cancer patients qualified for surgery.
Grenda, Anna; Wojas-Krawczyk, Kamila; Skoczylas, Tomasz; Krawczyk, Pawel; Sierocinska-Sawa, Jadwiga; Wallner, Grzegorz; Milanowski, Janusz.
Afiliação
  • Grenda A; Chair and Department of Pneumology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, 20-090, Lublin, Poland. an.grenda@gmail.com.
  • Wojas-Krawczyk K; Chair and Department of Pneumology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, 20-090, Lublin, Poland.
  • Skoczylas T; II Chair and Department of General and Gastrointestinal Surgery and Surgical Oncology of the Alimentary Tract, Medical University of Lublin, Staszica 16, 20-080, Lublin, Poland.
  • Krawczyk P; Chair and Department of Pneumology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, 20-090, Lublin, Poland.
  • Sierocinska-Sawa J; Laboratory of Pathomorphology, Independent Public Clinical Hospital No. 1 in Lublin, ul. Staszica 11, 20-081, Lublin, Poland.
  • Wallner G; II Chair and Department of General and Gastrointestinal Surgery and Surgical Oncology of the Alimentary Tract, Medical University of Lublin, Staszica 16, 20-080, Lublin, Poland.
  • Milanowski J; Chair and Department of Pneumology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, 20-090, Lublin, Poland.
BMC Gastroenterol ; 20(1): 382, 2020 Nov 16.
Article em En | MEDLINE | ID: mdl-33198632
ABSTRACT

BACKGROUND:

Amplification of HER2 gene (ERBB2) and overexpression of HER2 protein on cancer cells are found in 10-26% of gastric cancer (GC) and esophagogastric junction cancer (EGJC). Gene copy number variation (CNV) could be detected in these patients in liquid biopsy and in cancer cells.

METHODS:

We analysed HER2 gene CNV used qPCR method in 87 sera collected from GC and EGJC patients before surgical treatment and in 40 sera obtained from healthy donors. HER2 gene CNV was also assessed in formalin-fixed paraffin-embedded (FFPE) tumor tissue. Furthermore, we assessed the number of HER2 gene copies and HER2 expression in cancer cells using the fluorescent in situ hybridization method (FISH) and immunohistochemistry (IHC).

RESULTS:

We found that the HER2 gene copy number in liquid biopsy was higher in GC and EGJC patients compared to healthy people (p = 0.01). Moreover, EGJC patients had higher number of HER2 gene copies than healthy donors (p = 0.0016). HER2 CNV examination could distinguish healthy individuals and patients with gastric or esophagogastric junction cancers with sensitivity and specificity of 58% and 98% (AUC = 0.707, 95% CI 0.593-0.821, p = 0.004). We found that patients with a high copy number of the HER2 gene in the tumor tissue assessed by qPCR (but not by FISH) have significantly more often a high number of HER2 gene copies in liquid biopsy (p = 0.04).

CONCLUSIONS:

We suggested that HER2 testing in liquid biopsy could be used as an auxiliary method to analysis of HER2 status in tumor tissue in gastric or esophagogastric junction cancers.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Genes erbB-2 Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Genes erbB-2 Idioma: En Ano de publicação: 2020 Tipo de documento: Article