Effectiveness of a biodegradable 3D polylactic acid/poly(É-caprolactone)/hydroxyapatite scaffold loaded by differentiated osteogenic cells in a critical-sized radius bone defect in rat.
J Tissue Eng Regen Med
; 15(2): 150-162, 2021 02.
Article
em En
| MEDLINE
| ID: mdl-33216449
ABSTRACT
The effects of a scaffold made of polylactic acid, poly (É-caprolactone) and hydroxyapatite by indirect 3D printing method with and without differentiated bone cells was tested on the regeneration of a critical radial bone defect in rat. The scaffold characterization and mechanical performance were determined by the rheology, scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and Fourier transform infrared spectrometry. The defects were created in forty Wistar rats which were randomly divided into the untreated, autograft, scaffold cell-free, and differentiated bone cell-seeded scaffold groups (n = 10 in each group). The expression level of angiogenic and osteogenic markers, analyzed by quantitative real time-polymerase chain reaction (in vitro), significantly improved (p < 0.05) in the scaffold group compared to the untreated one. Radiology and computed tomography scan demonstrated a significant improvement in the cell-seeded scaffold group compared to the untreated one (p < 0.001). Biomechanical, histopathological, histomorphometric, and immunohistochemical investigations showed significantly better regeneration scores in the cell-seeded scaffold and autograft groups compared to the untreated group (p < 0.05). The cell-seeded scaffold and autograft groups did show comparable results on the 80th day post-treatment (p > 0.05), however, most results in the scaffold group were significantly higher than the untreated group (p < 0.05). Differentiated bone cells can enhance bone regeneration potential of the scaffold.
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Base de dados:
MEDLINE
Assunto principal:
Osteogênese
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Rádio (Anatomia)
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Células-Tronco
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Regeneração Óssea
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Células Imobilizadas
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Transplante de Células-Tronco
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Alicerces Teciduais
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article