dSTORM Imaging and Analysis of Desmosome Architecture.
Methods Mol Biol
; 2367: 305-315, 2021.
Article
em En
| MEDLINE
| ID: mdl-33225407
ABSTRACT
Desmosomes are cell-cell junctions responsible for mechanically integrating adjacent cells. Due to the small size of the junctions, their protein architecture cannot be elucidated using conventional fluorescence microscopy. Super-resolution microscopy techniques, including dSTORM, deliver higher-resolution images which can reveal the localization or arrangement of proteins within individual desmosomes. Herein we describe an imaging and analysis method to determine the nanoscale architecture of desmosomes using super-resolution dSTORM.
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Base de dados:
MEDLINE
Assunto principal:
Desmossomos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article