Role of Î³-H2AX as a biomarker for detection of bladder carcinogens in F344 rats.

Suzuki, Shugo; Gi, Min; Toyoda, Takeshi; Kato, Hiroyuki; Naiki-Ito, Aya; Kakehashi, Anna; Ogawa, Kumiko; Takahashi, Satoru; Wanibuchi, Hideki.

Afiliação

Suzuki S; Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan.
Gi M; Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, Aichi 467-8601, Japan.
Toyoda T; Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan.
Kato H; Department of Environmental Risk Assessment, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan.
Naiki-Ito A; Division of Pathology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki, Kanagawa 210-9501, Japan.
Kakehashi A; Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, Aichi 467-8601, Japan.
Ogawa K; Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, Aichi 467-8601, Japan.
Takahashi S; Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan.
Wanibuchi H; Division of Pathology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki, Kanagawa 210-9501, Japan.

J Toxicol Pathol ; 33(4): 279-285, 2020 Oct.

Article em En | MEDLINE | ID: mdl-33239845

ABSTRACT

Phosphorylation of histone H2AX at serine 139 (Î³-H2AX) is known to be induced by direct DNA damage or cellular metabolic imbalances and malfunctions. Previous studies have reported that Î³-H2AX is a useful biomarker for early detection of genotoxic bladder carcinogens in rats. The purpose of the present study was to determine the role of Î³-H2AX as a biomarker for detection of non-genotoxic bladder carcinogens in rats. Six-week-old male F344 rats were treated with 15 different chemicals for 4 weeks. Immunohistochemical analyses revealed that all three genotoxic bladder carcinogens and six out of seven non-genotoxic bladder carcinogens significantly increased Î³-H2AX formation in the bladder urothelium of rats. In addition, four out of five rat bladder noncarcinogens did not increase Î³-H2AX formation in the bladder urothelium regardless of genotoxicity. These results suggest that Î³-H2AX is a useful biomarker for detection of both genotoxic and non-genotoxic bladder carcinogens in rats.

Palavras-chave

F344 rats; carcinogen; prediction; urinary bladder; Î³-H2AX

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article