Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes.
Biomed Pharmacother
; 133: 111025, 2021 Jan.
Article
em En
| MEDLINE
| ID: mdl-33254018
ABSTRACT
The present study aimed to evaluate the antileishmanial effect, the mechanisms of action and the association with miltefosine of Vernonia brasiliana essential oil against Leishmania infantum promastigotes. This essential oil was obtained by hydrodistillation and its chemical composition was determined by gas chromatography-mass spectrometry (GC-MS). The antileishmanial activity against L. infantum promastigotes and cytotoxicity on DH82 cells were evaluated by MTT colorimetric assay. Ultrastructural alterations were evaluated by transmission electron microscopy. Changes in mitochondrial membrane potential, in the production of reactive oxygen species, and analysis of apoptotic events were determined by flow cytometry. The association between the essential oil and miltefosine was evaluated using the modified isobologram method. The most abundant component of the essential oil was ß-caryophyllene (21.47 %). Anti-Leishmania assays indicated an IC50 of 39.01⯱â¯1.080⯵g/mL for promastigote forms after 72â¯h of treatment. The cytotoxic concentration for DH82 cells was 63.13⯱â¯1.211⯵g/mL after 24â¯h of treatment. The effect against L. infantum was proven through the ultrastructural changes caused by the oil, such as kinetoplast and mitochondrial swelling, vesicles in the flagellar pocket, discontinuity of the nuclear membrane, nuclear fragmentation and condensation, and loss of organelles. It was observed that the oil leads to a decrease in the mitochondrial membrane potential (35.10 %, pâ¯=â¯0.0031), increased reactive oxygen species production, and cell death by late apoptosis (17.60 %, pâ¯=â¯0.020). The combination of the essential oil and miltefosine exhibited an antagonistic effect. This study evidences the antileishmanial action of V. brasiliana essential oil against L. infantum promastigotes.
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Base de dados:
MEDLINE
Assunto principal:
Óleos de Plantas
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Óleos Voláteis
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Apoptose
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Leishmania infantum
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Vernonia
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Sesquiterpenos Policíclicos
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Antiprotozoários
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article