Benzoic and salicylic acid are the signaling molecules of Chlorella cells for improving cell growth.
Chemosphere
; 265: 129084, 2021 Feb.
Article
em En
| MEDLINE
| ID: mdl-33261837
ABSTRACT
Cell-to-cell communication regulates microalgae production via signaling molecules (SMs), but few microalgal SM species are known. Here, we document two new microalgae SMs, benzoic acid (BA) and salicylic acid (SA). Initially, crude SMs were extracted from a microalgae culture in which microalgae grew on heterotrophic-enriched phosphorus nutrition. The extracted SMs enhanced Chlorella growth by â¼72%, promoted nutrient uptake, and up-regulated the mitogen-activated protein-kinase signaling cascade. Fourier transform infrared and nuclear magnetic resonance analyses identified the putative SMs was aromatic carboxylic acids. BA and SA were identified using high-resolution mass spectrometry. BA and SA addition increased cell growth by â¼75% and â¼25%; and improved ATP production by â¼35% and â¼20%. Transcriptomic analysis showed that BA and SA were biosynthesized via CoA-dependent, non-oxidative pathway. The SMs upregulated TCA-cycle enzymes, which promoted carbon assimilation and activated DNA-replicating enzyme, so that accelerated cell division. This study identified two new SMs for microalgae cell communication and provides means to identify other SMs.
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MEDLINE
Assunto principal:
Chlorella
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Microalgas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article