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Rapid single-molecule digital detection of protein biomarkers for continuous monitoring of systemic immune disorders.
Song, Yujing; Sandford, Erin; Tian, Yuzi; Yin, Qingtian; Kozminski, Andrew G; Su, Shiuan-Haur; Cai, Tao; Ye, Yuxuan; Chung, Meng Ting; Lindstrom, Ryan; Goicochea, Annika; Barabas, Jenny; Olesnavich, Mary; Rozwadowski, Michelle; Li, Yongqing; Alam, Hasan B; Singer, Benjamin H; Ghosh, Monalisa; Choi, Sung Won; Tewari, Muneesh; Kurabayashi, Katsuo.
Afiliação
  • Song Y; Department of Mechanical Engineering.
  • Sandford E; Department of Internal Medicine, Division of Hematology/Oncology, and.
  • Tian Y; Department of Surgery, University of Michigan, Ann Arbor, MI.
  • Yin Q; Department of Rheumatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
  • Kozminski AG; Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA; and.
  • Su SH; Department of Mechanical Engineering.
  • Cai T; Department of Mechanical Engineering.
  • Ye Y; Department of Mechanical Engineering.
  • Chung MT; Department of Mechanical Engineering.
  • Lindstrom R; Department of Mechanical Engineering.
  • Goicochea A; Department of Internal Medicine, Division of Hematology/Oncology, and.
  • Barabas J; Department of Internal Medicine, Division of Hematology/Oncology, and.
  • Olesnavich M; Department of Internal Medicine, Division of Hematology/Oncology, and.
  • Rozwadowski M; Department of Internal Medicine, Division of Hematology/Oncology, and.
  • Li Y; Department of Pediatrics.
  • Alam HB; Department of Surgery, University of Michigan, Ann Arbor, MI.
  • Singer BH; Department of Surgery, University of Michigan, Ann Arbor, MI.
  • Ghosh M; Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine.
  • Choi SW; Michigan Center for Integrative Research in Critical Care.
  • Tewari M; Department of Internal Medicine, Division of Hematology/Oncology, and.
  • Kurabayashi K; Department of Pediatrics.
Blood ; 137(12): 1591-1602, 2021 03 25.
Article em En | MEDLINE | ID: mdl-33275650
Digital protein assays have great potential to advance immunodiagnostics because of their single-molecule sensitivity, high precision, and robust measurements. However, translating digital protein assays to acute clinical care has been challenging because it requires deployment of these assays with a rapid turnaround. Herein, we present a technology platform for ultrafast digital protein biomarker detection by using single-molecule counting of immune-complex formation events at an early, pre-equilibrium state. This method, which we term "pre-equilibrium digital enzyme-linked immunosorbent assay" (PEdELISA), can quantify a multiplexed panel of protein biomarkers in 10 µL of serum within an unprecedented assay incubation time of 15 to 300 seconds over a 104 dynamic range. PEdELISA allowed us to perform rapid monitoring of protein biomarkers in patients manifesting post-chimeric antigen receptor T-cell therapy cytokine release syndrome, with ∼30-minute sample-to-answer time and a sub-picograms per mL limit of detection. The rapid, sensitive, and low-input volume biomarker quantification enabled by PEdELISA is broadly applicable to timely monitoring of acute disease, potentially enabling more personalized treatment.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Citocinas / Testes Imediatos / Doenças do Sistema Imunitário Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Citocinas / Testes Imediatos / Doenças do Sistema Imunitário Idioma: En Ano de publicação: 2021 Tipo de documento: Article