Rapid single-molecule digital detection of protein biomarkers for continuous monitoring of systemic immune disorders.
Blood
; 137(12): 1591-1602, 2021 03 25.
Article
em En
| MEDLINE
| ID: mdl-33275650
Digital protein assays have great potential to advance immunodiagnostics because of their single-molecule sensitivity, high precision, and robust measurements. However, translating digital protein assays to acute clinical care has been challenging because it requires deployment of these assays with a rapid turnaround. Herein, we present a technology platform for ultrafast digital protein biomarker detection by using single-molecule counting of immune-complex formation events at an early, pre-equilibrium state. This method, which we term "pre-equilibrium digital enzyme-linked immunosorbent assay" (PEdELISA), can quantify a multiplexed panel of protein biomarkers in 10 µL of serum within an unprecedented assay incubation time of 15 to 300 seconds over a 104 dynamic range. PEdELISA allowed us to perform rapid monitoring of protein biomarkers in patients manifesting post-chimeric antigen receptor T-cell therapy cytokine release syndrome, with â¼30-minute sample-to-answer time and a sub-picograms per mL limit of detection. The rapid, sensitive, and low-input volume biomarker quantification enabled by PEdELISA is broadly applicable to timely monitoring of acute disease, potentially enabling more personalized treatment.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Citocinas
/
Testes Imediatos
/
Doenças do Sistema Imunitário
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article