Genome-wide off-rates reveal how DNA binding dynamics shape transcription factor function.
Mol Syst Biol
; 16(10): e9885, 2020 10.
Article
em En
| MEDLINE
| ID: mdl-33280256
ABSTRACT
Protein-DNA interactions are dynamic, and these dynamics are an important aspect of chromatin-associated processes such as transcription or replication. Due to a lack of methods to study on- and off-rates across entire genomes, protein-DNA interaction dynamics have not been studied extensively. Here, we determine in vivo off-rates for the Saccharomyces cerevisiae chromatin organizing factor Abf1, at 191 sites simultaneously across the yeast genome. Average Abf1 residence times span a wide range, varying between 4.2 and 33 min. Sites with different off-rates are associated with different functional characteristics. This includes their transcriptional dependency on Abf1, nucleosome positioning and the size of the nucleosome-free region, as well as the ability to roadblock RNA polymerase II for termination. The results show how off-rates contribute to transcription factor function and that DIVORSEQ (Determining In Vivo Off-Rates by SEQuencing) is a meaningful way of investigating protein-DNA binding dynamics genome-wide.
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MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Fatores de Transcrição
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Transcrição Gênica
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Cromatina
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Nucleossomos
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Proteínas de Saccharomyces cerevisiae
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Proteínas de Ligação a DNA
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Sequenciamento de Cromatina por Imunoprecipitação
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article