3D-Non-destructive Imaging through Heavy-Metal Eosin Salt Contrast Agents.
Chemistry
; 27(14): 4561-4566, 2021 Mar 08.
Article
em En
| MEDLINE
| ID: mdl-33300642
ABSTRACT
Conventional histology is a destructive technique based on the evaluation of 2D slices of a 3D biopsy. By using 3D X-ray histology these obstacles can be overcome, but their application is still restricted due to the inherently low attenuation properties of soft tissue. In order to solve this problem, the tissue can be stained before X-ray computed tomography imaging (CT) to enhance the soft tissue X-ray contrast. Evaluation of brominated fluorescein salts revealed a mutual influence of the number of bromine atoms and the cations applied on the achieved contrast enhancement. The dibromo fluorescein barium salt turned out to be the ideal X-ray contrast agent, allowing for 3D imaging and subsequent complementing counterstaining applying standard histological techniques.
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Base de dados:
MEDLINE
Assunto principal:
Meios de Contraste
/
Imageamento Tridimensional
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article