Novel near-infrared fluorescence probe with large Stokes shift for monitoring CCl4-induced toxic hepatitis.
Talanta
; 223(Pt 1): 121720, 2021 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-33303166
ABSTRACT
Toxic hepatitis which is induced by chemical substance is a serious threat to human health. More and more studies have shown that peroxynitrite (ONOO-) is related with the development of toxic hepatitis. So it is important to find a tool to study ONOO- change during the diagnosis and therapy of toxic hepatitis. Herein, a series of novel near-infrared (NIR) fluorescence dyes (DDM-R) with long emission wavelength (740-770 nm) and large Stokes shift (~200 nm) are developed. Among the dyes, DDM-OH with great spectral performance and facilely modified feature is used to construct probe DDM-ONOO-. The probe have the preference of high sensitivity and excellent selectivity for ONOO-. In addition, DDM-ONOO- was applied in detecting exogenous and endogenous ONOO- in cells and further used in detecting ONOO- of CCl4-induced toxic hepatitis in cells by fluorescence imaging, 3D quantification analysis, flow cytometry. More importantly, by visualizing ONOO-, the probe was used to monitor the diagnosis of CCl4-induced toxic hepatitis in mice and evaluate the therapeutic efficacy of hepatoprotective medicines (NAC, SM, DDB). The results show that the probe will provide a powerful tool for the diagnosis and treatment of toxic hepatitis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ácido Peroxinitroso
/
Doença Hepática Induzida por Substâncias e Drogas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article