An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification.

Cai, Hongmin; Yao, Hebang; Li, Tingting; Hutter, Cedric A J; Li, Yanfang; Tang, Yannan; Seeger, Markus A; Li, Dianfan

Cai, Hongmin; Yao, Hebang; Li, Tingting; Hutter, Cedric A J; Li, Yanfang; Tang, Yannan; Seeger, Markus A; Li, Dianfan.

Afiliação

Cai H; University of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, 200031, Shanghai, China.
Yao H; University of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, 200031, Shanghai, China.
Li T; University of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, 200031, Shanghai, China.
Hutter CAJ; Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
Li Y; University of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, 200031, Shanghai, China.
Tang Y; University of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, 200031, Shanghai, China.
Seeger MA; Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
Li D; University of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, 200031, Shanghai, China. dianfan.li@sibcb.ac.cn.

Commun Biol ; 3(1): 753, 2020 12 10.

Article em En | MEDLINE | ID: mdl-33303987

Assuntos

Proteínas de Fluorescência Verde/química; Proteínas de Membrana/genética; Proteínas de Membrana/isolamento & purificação; Anticorpos de Domínio Único/química; Cromatografia de Afinidade; Escherichia coli/genética; Escherichia coli/metabolismo; Expressão Gênica; Genes Reporter; Proteínas Luminescentes; Proteínas de Membrana/química; Modelos Moleculares; Conformação Proteica; Estabilidade Proteica; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/isolamento & purificação; Proteínas Recombinantes/metabolismo; Saccharomyces cerevisiae/genética; Saccharomyces cerevisiae/metabolismo

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Fluorescência Verde / Anticorpos de Domínio Único / Proteínas de Membrana Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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