RNA-Centric Methods: Toward the Interactome of Specific RNA Transcripts.
Trends Biotechnol
; 39(9): 890-900, 2021 09.
Article
em En
| MEDLINE
| ID: mdl-33353763
RNA-protein interactions play an important role in numerous cellular processes in health and disease. In recent years, the global RNA-bound proteome has been extensively studied, uncovering many previously unknown RNA-binding proteins. However, little is known about which particular proteins bind to which specific RNA transcript. In this review, we provide an overview of methods to identify RNA-protein interactions, with a particular focus on strategies that provide insights into the interactome of specific RNA transcripts. Finally, we discuss challenges and future directions, including the potential of CRISPR-RNA targeting systems to investigate endogenous RNA-protein interactions.
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1
Base de dados:
MEDLINE
Assunto principal:
RNA
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Proteínas de Ligação a RNA
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Biologia Computacional
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article