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Peptide ELISA and FRET-qPCR Identified a Significantly Higher Prevalence of Chlamydia suis in Domestic Pigs Than in Feral Swine from the State of Alabama, USA.
Hoque, Md Monirul; Adekanmbi, Folasade; Barua, Subarna; Rahman, Kh Shamsur; Aida, Virginia; Anderson, Brian; Poudel, Anil; Kalalah, Anwar; Bolds, Sara; Madere, Steven; Kitchens, Steven; Price, Stuart; Brown, Vienna; Lockaby, B Graeme; Kyriakis, Constantinos S; Kaltenboeck, Bernhard; Wang, Chengming.
Afiliação
  • Hoque MM; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Adekanmbi F; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Barua S; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Rahman KS; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Aida V; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Anderson B; Swine Research and Education Center, Auburn University, Auburn, AL 36830, USA.
  • Poudel A; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Kalalah A; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Bolds S; School of Forestry and Wildlife Sciences, Auburn University, Auburn, AL 36849, USA.
  • Madere S; School of Forestry and Wildlife Sciences, Auburn University, Auburn, AL 36849, USA.
  • Kitchens S; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Price S; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Brown V; National Feral Swine Damage Management Program, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, CO 80521, USA.
  • Lockaby BG; School of Forestry and Wildlife Sciences, Auburn University, Auburn, AL 36849, USA.
  • Kyriakis CS; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Kaltenboeck B; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
  • Wang C; College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
Pathogens ; 10(1)2020 Dec 25.
Article em En | MEDLINE | ID: mdl-33375583
ABSTRACT
Chlamydia suis is an important, highly prevalent, and diverse obligate intracellular pathogen infecting pigs. In order to investigate the prevalence and diversity of C. suis in the U.S., 276 whole blood samples from feral swine were collected as well as 109 fecal swabs and 60 whole blood samples from domestic pigs. C. suis-specific peptide ELISA identified anti-C. suis antibodies in 13.0% of the blood of feral swine (26/276) and 80.0% of the domestic pigs (48/60). FRET-qPCR and DNA sequencing found C. suis DNA in 99.1% of the fecal swabs (108/109) and 21.7% of the whole blood (13/60) of the domestic pigs, but not in any of the assayed blood samples (0/267) in feral swine. Phylogenetic comparison of partial C. suis ompA gene sequences and C. suis-specific multilocus sequencing typing (MLST) revealed significant genetic diversity of the C. suis identified in this study. Highly genetically diverse C. suis strains are prevalent in domestic pigs in the USA. As crowding strongly enhances the frequency and intensity of highly prevalent Chlamydia infections in animals, less population density in feral swine than in domestic pigs may explain the significantly lower C. suis prevalence in feral swine. A future study is warranted to obtain C. suis DNA from feral swine to perform genetic diversity of C. suis between commercial and feral pigs.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article