Protocol for High-Throughput Analysis of Sister-Chromatids Contacts.
STAR Protoc
; 1(3): 100202, 2020 12 18.
Article
em En
| MEDLINE
| ID: mdl-33377096
Sister chromatid interactions are a key step to ensure the successful segregation of sister chromatids after replication. Our knowledge about this phenomenon is mostly based on microscopy approaches, which have some constraints such as resolution limit and the impossibility of studying several genomic positions at the same time. Here, we present a protocol for Hi-SC2, a high-throughput sequencing-based method, to monitor sister chromatid contacts after replication at high resolution throughout the genome, which we applied to study cohesion in Vibrio cholerae. For complete details on the use and execution of this protocol, please refer to Espinosa et al. (2020).
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Base de dados:
MEDLINE
Assunto principal:
Troca de Cromátide Irmã
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Análise de Sequência de DNA
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Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article