Purification and Use of tRNA for Enzymatic Post-translational Addition of Amino Acids to Proteins.
STAR Protoc
; 1(3): 100207, 2020 12 18.
Article
em En
| MEDLINE
| ID: mdl-33377101
ABSTRACT
Post-translational addition of amino acids to proteins by enzymes using aminoacyl-tRNA is an emerging regulatory mechanism. Examples include Arg transfer in eukaryotes, Leu/Phe transfer in bacteria, and tRNA-synthetase-mediated addition of amino acids to Lys side chains. Here, we present a method of purification and use of tRNA for such reactions, focusing on tRNAArg and its use for arginylation. This method can also be used for other tRNA-mediated reactions. For complete details on the use and execution of this protocol, please refer to Avcilar-Kucukgoze et al. (2020).
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Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência
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Processamento de Proteína Pós-Traducional
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Ensaios Enzimáticos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article