Discovery of Thanafactin A, a Linear, Proline-Containing Octalipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining.
J Nat Prod
; 84(1): 101-109, 2021 01 22.
Article
em En
| MEDLINE
| ID: mdl-33382250
ABSTRACT
Genome mining of the bacterial strains Pseudomonas sp. SH-C52 and Pseudomonas fluorescens DSM 11579 showed that both strains contained a highly similar gene cluster encoding an octamodular nonribosomal peptide synthetase (NRPS) system which was not associated with a known secondary metabolite. Insertional mutagenesis of an NRPS component followed by comparative profiling led to the discovery of the corresponding novel linear octalipopeptide thanafactin A, which was subsequently isolated and its structure determined by two-dimensional NMR and further spectroscopic and chromatographic methods. In bioassays, thanafactin A exhibited weak protease inhibitory activity and was found to modulate swarming motility in a strain-specific manner.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeo Sintases
/
Pseudomonas
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Prolina
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article