Detection, quantification and genotype distribution of HCV patients in Lahore, Pakistan by real-time PCR.
Afr Health Sci
; 20(3): 1143-1152, 2020 Sep.
Article
em En
| MEDLINE
| ID: mdl-33402959
ABSTRACT
BACKGROUND:
Hepatitis C virus (HCV) is considered as "Viral Time Bomb" suggested by the World Health Organization and if it is not treated timely, it will lead towards cirrhosis and hepatocellular carcinoma (HCC).OBJECTIVE:
The purpose of the present research is to study possible risk factors, frequent genotypes of HCV and its association with different age groups.METHODS:
Suspected blood samples from HCV patients were collected from different hospitals of Lahore, Pakistan. Out of 1000 HCV suspected samples, 920 samples were found HCV positive detected by Anti-HCV ELISA, CobasR. kit. The quantification of HCV load was determined by HCV quantification kit and LINEAR ARRAY KIT (Roche) was used for genotype determination by Real-Time PCR (ABI). Statistical analysis was done by using Microsoft Excel.RESULTS:
Out of 920 subjects, 77 subjects (8.4%) were false positive and they were not detected by nested PCR. Three PCR positive samples were untypeable. Genotype 3 was predominant in Lahore which was 83.5%, whereas type 1 and 2 were 5.1% and 0.7% respectively. There were also mixed genotypes detected, 1 and 3 were 0.4%, 2 and 3 were 1.41% and 3 and 4 were 0.2% only. Male were more infected of HCV in the age <40 years and females >40years.CONCLUSION:
The major risk factor for HCV transmission is by use of unsterilized razors/blades. It is necessary to spread awareness among the general population of Pakistan about HCV transmission risk factors. Regular physical examination at least once a year is recommended, so that early detection of HCV could be done.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Hepatite C
/
Hepacivirus
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Carcinoma Hepatocelular
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Hepatopatias
/
Neoplasias Hepáticas
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article