Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell.
Biosensors (Basel)
; 11(1)2021 Jan 07.
Article
em En
| MEDLINE
| ID: mdl-33430194
ABSTRACT
Biofuel cells allow for constructing sensors that leverage the specificity of enzymes without the need for an external power source. In this work, we design a self-powered glucose sensor based on a biofuel cell. The redox enzymes glucose dehydrogenase (NAD-GDH), glucose oxidase (GOx), and horseradish peroxidase (HRP) were immobilized as biocatalysts on the electrodes, which were previously engineered using carbon nanostructures, including multi-wall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO). Additional polymers were also introduced to improve biocatalyst immobilization. The reported design offers three main advantages (i) by using glucose as the substrate for the both anode and cathode, a more compact and robust design is enabled, (ii) the system operates under air-saturating conditions, with no need for gas purge, and (iii) the combination of carbon nanostructures and a multi-enzyme cascade maximizes the sensitivity of the biosensor. Our design allows the reliable detection of glucose in the range of 0.1-7.0 mM, which is perfectly suited for common biofluids and industrial food samples.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Nanotubos de Carbono
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Enzimas Imobilizadas
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Glucose
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article